Flow cytometry ALL  

 

    pro-B-ALL common-B-ALL pre-B-ALL transitional pre-B-ALL
  CD19 ++ ++ ++ ++
  cyCD79a ++ ++ ++ ++
  HLA-Dr ++ ++ ++ ++
  TdT ++ ++ (+) (+)
  CD34 + (+) (+)
  CD10 ++ ++ ++
  CD20 (+) (+) +
  cyIgM ++ ++
  surfIgM ++

 

    pro-T-ALL immature T-ALL common T-ALL mature T-ALL
  TdT ++ ++ ++ ++
  CD34 ++
  HLA-Dr ++
  CD1a +
  CD2 + ++ ++ ++
  cyCD3 ++ ++ ++ ++
  surfCD3 +/- ++
  CD5 ++ ++ ++
  CD7 ++ ++ ++ ++
  CD4-/CD8- ++ +
  CD4+/CD8- +/- +/- +
  CD4-/CD8+ +/- +/- +/-
  CD4+/CD8+ + + +/-
  CD10 +

 

– = < 10% positivity is found in these cases
± = 10-25% positivity is found in these cases
+ = 25-75% positivity is found in these cases
++ = > 75% positivity is found in these cases