Demo 1: Different morphology of megakaryocytes to help and guide to diagnosis
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Normal megakaryocytes: Strongly multi-lobulated nucleus with irregular lobes, connected with chromatin threads, with a clumped chromatin structure. Cytoplasma can be anything from basophilic to pink and can contain from none to abundant azurophilic granules |
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Micromegakaryocytes: mononuclear cell 12-40 µm diameter, sometimes bilobulated, dense chromatin with high nucleas to cytoplasm ratio. Cytoplasm is moderately mature |
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Hypolobulation: normal sized megakaryocyte with hypo- or non-lobulated nucleus, rond to oval shaped, normal chromatin condensation and normal cytoplasm structure. |
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Multilobulation: normal to large megakaryocyte with multiple separate nuclei, usually rond, normal to dense chromatin condensation and normal cytoplasm structure. |
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CML: Increased number of smaller megakaryocytes and sometimes hypolobulated. |
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PV: Increased number of morphologically normal megakaryocytes, in all different maturation stages. Often clustering. |
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ET: Many megakaryocyten in normocellulair bonemarrow, with bizarre giant megakaryocytes, with abundant cytoplasm and hyperlobulated nucleus (stag-horn shaped) and clumped chromatin. |
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Osteoclasts: Large cells, have multinucleated, well-separated and uniformly sized round to ovoid nuclei. |
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Plasmacells: Multilobulated giant plasmacells |
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Morbus Hodgkin: Hodgkin cells: large multilobulated nucleus with coarse chromatin, multiple bluish nucleoli, abundant slightly basophilic cytoplasm. |